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Frozen Sperm

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Home > Fertility > Male Fertility Treatments > Frozen Sperm

Frozen Sperm - Sperm Freezing in India

Instructions and Method of Collection of Sperm

If possible, you will be requested a period of 3-5 days of abstinence from ejaculation before producing a specimen for storage. It usually takes males 3-5 days to produce a semen sample with optimal sperm count (the quality of the sperm may deteriorate over shorter or longer periods of abstinence).

It is preferred that the specimen be obtained by manual stimulation (masturbation). There are private rooms at the facility that may be used for this purpose. If you choose to produce the sample at home, please do not use any lubricants as these products may compromise the quality of the sample. The sample must be collected in a sterile container, which you may obtain from our laboratory, and brought to IDANT within 60 minutes from production. It is essential that the specimen be kept at body temperature during transit.


To ensure accurate identification, an accession number and a can number is assigned to his specimen from the moment it is received. A complete semen analysis is then performed. This includes semen volume, liquefaction and viscosity, sperm count, motility, forward progression, and morphology.

The specimen is then processed either as intracervical insemination (ICI) units or intrauterine insemination (IUI) units and stored in 0.5 ml sterile straws. Each straw is labeled with his name, social security number, date, accession number, and can number. A small portion of his specimen is drawn into a separate straw for a post- thaw analysis, which is performed the following business day to assess your specimens cryosurvival. The straws are then placed in a metal storage canister labeled identically and stored under liquid nitrogen.


All client-depositors are required to be tested for a number of infectious diseases as required by licensing and accrediting agencies. A technician will draw blood at the time of the initial visit. Screening will be performed by a licensed independent laboratory routinely used by the Idant Laboratories.

The required screening includes HIV-1, HIV-2, HTLV-I, HTLV-II, Hepatitis B surface Antigen, Hepatitis B core Antibody, Hepatitis C Viral Antibody, Syphilis, CMV, and Gonorrhea Culture.

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